The Applied Molecular Biotechnology Laboratory (AMBL) is a requisite course for the MS concentration in Applied Molecular Biology. AMBL combines hands on laboratory training with independent research experience to train students in the latest techniques and concepts of molecular biology and biochemistry. Recombinant DNA technology is the primary focus of the first semester. Topics covered include nucleic acid isolation, genomics, quantitative PCR and RT-PCR, southern and northern blot analysis, and computational biological analyses. Protein expression, purification, and detection are the focus of the second semester. Topics covered include prokaryotic and eukaryotic protein expression systems, column chromatography, proteomics, western blot analysis, enzymatic assay, and microscopic analysis. Throughout the year-long course, students will develop critical thinking skills and gain valuable research experience, all while working on real world problems and preparing for careers in the biotechnology and pharmaceutical industries.