@article {651, title = {Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals.}, journal = {Arch Microbiol}, volume = {159}, year = {1993}, month = {1993}, pages = {336-44}, abstract = {The gram-negative metal-reducing microorganism, previously known as strain GS-15, was further characterized. This strict anaerobe oxidizes several short-chain fatty acids, alcohols, and monoaromatic compounds with Fe(III) as the sole electron acceptor. Furthermore, acetate is also oxidized with the reduction of Mn(IV), U(VI), and nitrate. In whole cell suspensions, the c-type cytochrome(s) of this organism was oxidized by physiological electron acceptors and also by gold, silver, mercury, and chromate. Menaquinone was recovered in concentrations comparable to those previously found in gram-negative sulfate reducers. Profiles of the phospholipid ester-linked fatty acids indicated that both the anaerobic desaturase and the branched pathways for fatty acid biosynthesis were operative. The organism contained three lipopolysaccharide hydroxy fatty acids which have not been previously reported in microorganisms, but have been observed in anaerobic freshwater sediments. The 16S rRNA sequence indicated that this organism belongs in the delta proteobacteria. Its closest known relative is Desulfuromonas acetoxidans. The name Geobacter metallireducens is proposed.}, keywords = {Cytochrome c Group, Fatty Acids, Gram-Negative Anaerobic Bacteria, Iron, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S}, issn = {0302-8933}, author = {Lovley, D R and Giovannoni, S J and White, D C and Champine, J E and Phillips, E J and Gorby, Y A and Goodwin, S} } @article {872, title = {Acetate catabolism in the dissimilatory iron-reducing isolate GS-15.}, journal = {J Bacteriol}, volume = {173}, year = {1991}, month = {1991 Apr}, pages = {2704-6}, abstract = {Acetate-grown GS-15 whole-cell suspensions were disrupted with detergent and assayed for enzymes associated with acetate catabolism. Carbon monoxide dehydrogenase and formate dehydrogenase were not observed in GS-15. Catabolic levels of acetokinase and phosphotransacetylase were observed. Enzyme activities of the citric acid cycle, i.e., isocitrate dehydrogenase, 2-oxoglutarate sythase, succinate dehydrogenase, fumarase, and malate dehydrogenase, were observed.}, keywords = {Acetate Kinase, Acetates, Acetyl Coenzyme A, Bacteria, Carbon Monoxide, Citric Acid Cycle, Electron Transport, Euryarchaeota, Fumarate Hydratase, Isocitrate Dehydrogenase, Ketone Oxidoreductases, Malate Dehydrogenase, Phosphate Acetyltransferase, Pseudomonas aeruginosa, Succinate Dehydrogenase}, issn = {0021-9193}, author = {Champine, J E and Goodwin, S} }