@article {356, title = {Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.}, journal = {Viruses}, volume = {4}, year = {2012}, month = {2012 Jan}, pages = {28-61}, abstract = {The sexually transmitted insect virus Helicoverpa zea nudivirus 2 (HzNV-2) was determined to have a circular double-stranded DNA genome of 231,621 bp coding for an estimated 113 open reading frames (ORFs). HzNV-2 is most closely related to the nudiviruses, a sister group of the insect baculoviruses. Several putative ORFs that share homology with the baculovirus core genes were identified in the viral genome. However, HzNV-2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, LEF-1 and the palindromic hrs, which serve as origins of replication. The HzNV-2 genome was found to code for three ORFs that had significant sequence homology to cellular genes which are not generally found in viral genomes. These included a presumed juvenile hormone esterase gene, a gene coding for a putative zinc-dependent matrix metalloprotease, and a major facilitator superfamily protein gene; all of which are believed to play a role in the cellular proliferation and the tissue hypertrophy observed in the malformation of reproductive organs observed in HzNV-2 infected corn earworm moths, Helicoverpa zea.}, keywords = {Amino Acid Sequence, Animals, Baculoviridae, Base Sequence, Biological Evolution, Consensus Sequence, DNA Viruses, Female, Genome, Viral, Insect Viruses, Molecular Sequence Data, Moths, Open Reading Frames, Phylogeny, Sequence Alignment, Sequence Homology, Species Specificity, Viral Proteins}, issn = {1999-4915}, doi = {10.3390/v4010028}, author = {Burand, John P and Kim, Woojin and Afonso, Claudio L and Tulman, Edan R and Kutish, Gerald F and Lu, Zhiqiang and Rock, Daniel L} } @article {361, title = {Infection with the insect virus Hz-2v alters mating behavior and pheromone production in female Helicoverpa zea moths.}, journal = {J Insect Sci}, volume = {5}, year = {2005}, month = {2005}, pages = {6}, abstract = {The effect of Hz-2V virus infection on the reproductive physiology and behavior of infected Helicoverpa zea female moths was examined. In the absence of males, infected females exhibited calling behavior and called as often but for shorter periods on average than control females. As expected, control females mated with males for extend periods when they were present and did not call after mating, while virus-infected females made many frequent contacts with males and continued to call even after these contacts. Virus-infected females were found to produce five to seven times more pheromone than control females and attracted twice as many males as did control females in flight tunnel experiments. The ability of Hz-2V to alter the physiology and behavior of infected females observed here may serve to facilitate the transmission of virus in insect populations.}, keywords = {Animals, Female, Infectious Disease Transmission, Vertical, Insect Viruses, Male, Moths, Pheromones, Sexual Behavior, Animal, Time Factors, Vocalization, Animal}, issn = {1536-2442}, author = {Burand, John P and Tan, Weijia and Kim, Woojin and Nojima, Satoshi and Roelofs, Wendell} }