Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis.

TitleExtensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis.
Publication TypeJournal Article
Year of Publication2000
AuthorsFeng X, Rich SM, Akiyoshi D, Tumwine JK, Kekitiinwa A, Nabukeera N, Tzipori S, Widmer G
JournalAppl Environ Microbiol
Date Published2000 Aug
KeywordsAnimals, Base Sequence, Cattle, Cryptosporidiosis, Cryptosporidium parvum, DNA, Protozoan, Humans, Karyotyping, Mice, Mice, Knockout, Microsatellite Repeats, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Genetic, Sequence Analysis, DNA

Restriction fragment length polymorphism and DNA sequence analysis discern two main types of Cryptosporidium parvum. We present a survey of length polymorphism at several microsatellite loci for type 1 and type 2 isolates. A total of 14 microsatellite loci were identified from C. parvum DNA sequences deposited in public databases. All repeats were mono-, di-, and trinucleotide repeats of A, AT, and AAT, reflecting the high AT content of the C. parvum genome. Several of these loci showed significant length polymorphism, with as many as seven alleles identified for a single locus. Differences between alleles ranged from 1 to 27 bp. Karyotype analysis using probes flanking three microsatellites localized each marker to an individual chromosomal band, suggesting that these markers are single copy. In a sample of 19 isolates for which at least three microsatellites were typed, a majority of isolates displayed a unique multilocus fingerprint. Microsatellite analysis of isolates passaged between different host species identified genotypic changes consistent with changes in parasite populations.

Alternate JournalAppl. Environ. Microbiol.
PubMed ID10919789