This course teaches the fundamental methodology relevant to the practice of modern biotechnology. Techniques include the amplification, manipulation, isolation, and analysis of DNA, the production of recombinant DNA (vectors) for cloning and protein expression, as well as the establishment of genetically modified cell lines via different selection and screening methods. Further principles taught are isolation and characterization of proteins, and use of immunoassays. Overall, a major emphasis is laid on the student's individual performance of laboratory exercises, which provides direct, active laboratory experience.
MICROBIO 312 with a C or higher
Department of Microbiology