Molecular analysis of the recF gene of Escherichia coli.

TitleMolecular analysis of the recF gene of Escherichia coli.
Publication TypeJournal Article
Year of Publication1984
AuthorsBlanar MA, Sandler SJ, Armengod ME, Ream LW, Clark AJ
JournalProc Natl Acad Sci U S A
Date Published1984 Aug
KeywordsAmino Acid Sequence, Bacterial Proteins, Base Sequence, DNA Repair, DNA Replication, Escherichia coli, Genes, Bacterial, Molecular Weight, Mutation, Plasmids, Recombination, Genetic

We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations.

Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID6379647