Complete genome sequence of "Enterobacter lignolyticus" SCF1.

TitleComplete genome sequence of "Enterobacter lignolyticus" SCF1.
Publication TypeJournal Article
Year of Publication2011
AuthorsDeangelis KM, D'haeseleer P, Chivian D, Fortney JL, Khudyakov J, Simmons B, Woo H, Arkin AP, Davenport K W, Goodwin L, Chen A, Ivanova N, Kyrpides NC, Mavromatis K, Woyke T, Hazen TC
JournalStand Genomic Sci
Volume5
Issue1
Pagination69-85
Date Published2011 Oct 15
ISSN1944-3277
Abstract

In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated "Enterobacter lignolyticus" SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of "E. lignolyticus" SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster.

DOI10.4056/sigs.2104875
Alternate JournalStand Genomic Sci
PubMed ID22180812