Differences in Fe(III) reduction in the hyperthermophilic archaeon, Pyrobaculum islandicum, versus mesophilic Fe(III)-reducing bacteria.

TitleDifferences in Fe(III) reduction in the hyperthermophilic archaeon, Pyrobaculum islandicum, versus mesophilic Fe(III)-reducing bacteria.
Publication TypeJournal Article
Year of Publication2001
AuthorsChilders SE, Lovley DR
JournalFEMS Microbiol Lett
Volume195
Issue2
Pagination253-8
Date Published2001 Feb 20
ISSN0378-1097
KeywordsCytochrome c Group, Electron Transport, Ferric Compounds, FMN Reductase, NADH, NADPH Oxidoreductases, NADP, Oxidation-Reduction, Temperature, Thermoproteaceae
Abstract

The discovery that all hyperthermophiles that have been evaluated have the capacity to reduce Fe(III) has raised the question of whether mechanisms for dissimilatory Fe(III) reduction have been conserved throughout microbial evolution. Many studies have suggested that c-type cytochromes are integral components in electron transport to Fe(III) in mesophilic dissimilatory Fe(III)-reducing microorganisms. However, Pyrobaculum islandicum, the hyperthermophile in which Fe(III) reduction has been most intensively studied, did not contain c-type cytochromes. NADPH was a better electron donor for the Fe(III) reductase activity in P. islandicum than NADH. This is the opposite of what has been observed with mesophiles. Thus, if previous models for dissimilatory Fe(III) reduction by mesophilic bacteria are correct, then it is unlikely that a single strategy for electron transport to Fe(III) is present in all dissimilatory Fe(III)-reducing microorganisms.

Alternate JournalFEMS Microbiol. Lett.
PubMed ID11179660