recO and recR mutations delay induction of the SOS response in Escherichia coli.

TitlerecO and recR mutations delay induction of the SOS response in Escherichia coli.
Publication TypeJournal Article
Year of Publication1995
AuthorsHegde S, Sandler SJ, Clark AJ, Madiraju MV
JournalMol Gen Genet
Volume246
Issue2
Pagination254-8
Date Published1995 Jan 20
ISSN0026-8925
KeywordsBacterial Proteins, beta-Galactosidase, DNA-Binding Proteins, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Mutation, Promoter Regions, Genetic, Recombinant Fusion Proteins, Repressor Proteins, Serine Endopeptidases, SOS Response (Genetics), Ultraviolet Rays
Abstract

RecF, RecO and RecR, three of the important proteins of the RecF pathway of recombination, are also needed for repair of DNA damage due to UV irradiation. recF mutants are not proficient in cleaving LexA repressor in vivo following DNA damage: therefore they show a delay of induction of the SOS response. In this communication, by measuring the in vivo levels of LexA repressor using anti-LexA antibodies, we show that recO and recR mutant strains are also not proficient in LexA cleavage reactions. In addition, we show that recO and recR mutations delay induction of beta-galactosidase activity expressed from a lexA-regulated promoter following exposure of cells to UV, thus further supporting the idea that recF, recO and recR gene products are needed for induction of the SOS response.

Alternate JournalMol. Gen. Genet.
PubMed ID7862097