Decorating the Outer Surface of Microbially Produced Protein Nanowires with Peptides.

TitleDecorating the Outer Surface of Microbially Produced Protein Nanowires with Peptides.
Publication TypeJournal Article
Year of Publication2019
AuthorsUeki T, Walker DJF, Tremblay P-L, Nevin KP, Ward JE, Woodard TL, Nonnenmann SS, Lovley DR
JournalACS Synth Biol
Date Published2019 Aug 16
KeywordsCarboxy-Lyases, Ethylene Glycols, Molecular Structure, Nanowires, Oxygenases, Peptides, Phenylalanine Ammonia-Lyase, Plasmids, Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Styrenes

The potential applications of electrically conductive protein nanowires (e-PNs) harvested from might be greatly expanded if the outer surface of the wires could be modified to confer novel sensing capabilities or to enhance binding to other materials. We developed a simple strategy for functionalizing e-PNs with surface-exposed peptides. The gene for the monomer that assembles into e-PNs was modified to add peptide tags at the carboxyl terminus of the monomer. Strains of were constructed that fabricated synthetic e-PNs with a six-histidine "His-tag" or both the His-tag and a nine-peptide "HA-tag" exposed on the outer surface. Addition of the peptide tags did not diminish e-PN conductivity. The abundance of HA-tag in e-PNs was controlled by placing expression of the gene for the synthetic monomer with the HA-tag under transcriptional regulation. These studies suggest broad possibilities for tailoring e-PN properties for diverse applications.

Alternate JournalACS Synth Biol
PubMed ID31298834